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Gentamicin used as an adjunct to GTR
Author(s) -
Stavropoulos A.,
Kostopoulos L.,
Mardas N.,
Nyengaard J. R.,
Karring T.
Publication year - 2003
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2003.10259.x
Subject(s) - capsule , gentamicin , connective tissue , medicine , dentistry , anatomy , biomedical engineering , chemistry , pathology , biology , antibiotics , biochemistry , botany
Abstract Objectives: To evaluate in a discriminating “capsule” model whether local application of gentamicin may have an added effect on bone formation produced by Bio‐Oss ® and guide tissue regeneration (GTR). Material and Methods: Thirty male 3‐month‐old Wistar rats were used. After elevation of muscle‐periosteal flaps, a rigid hemispherical Teflon capsule, loosely packed with 0.025 g of Bio‐Oss ® impregnated with 2 mg/ml gentamicin sulfate (Garamycin ® ), was placed with its open part facing the lateral bone surface of the mandibular ramus (test) in one side of the jaw. A capsule filled only with Bio‐Oss ® (control) was placed on the contralateral side of the jaw. After healing periods of 1, 2 and 4 months, groups of 10 animals were sacrificed and the specimens were processed for histological examination. The volumes of (1) the space created by the capsule, (2) newly formed bone, (3) Bio‐Oss ® particles, (4) loose connective tissue, and (5) acellular space in the capsule were estimated by a point‐counting technique in three to four histological sections of each specimen, taken by uniformly random sampling. Results: The histological evaluation showed limited but increasing bone fill in the capsules from 1 to 4 months in both the test and control sides. After 4 months, the newly formed bone occupied 11.9% (CV: 0.39) of the space created by the capsules at the test sides versus 13.2% (CV: 0.41) at the control sides. There was no statistical significant difference between test and control specimens at any observation time ( p >0.05). Conclusion: It is concluded that local application of gentamicin has no added effect on bone formation when combined with Bio‐Oss ® and GTR.