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Effects of enamel matrix protein application on the viability, proliferation, and attachment of human periodontal ligament fibroblasts to diseased root surfaces in vitro
Author(s) -
Davenport D. R.,
Mailhot J. M.,
Wataha J. C.,
Billman M. A.,
Sharawy M. M.,
Shrout M. K.
Publication year - 2003
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2003.00150.x
Subject(s) - periodontal fiber , viability assay , amelogenin , in vitro , andrology , enamel paint , dentin , matrix (chemical analysis) , cell growth , microbiology and biotechnology , chemistry , biology , dentistry , immunology , biochemistry , medicine , chromatography
Objectives: The purpose of this research was to examine the influence of enamel matrix proteins (EMP) on the viability, proliferation, and attachment of periodontal ligament fibroblasts (PDLF) to diseased root surfaces. Materials and Methods: Primary cell cultures of PDFL were obtained from clinically healthy third molars or premolar teeth. Viability and proliferation rates were carried out over a 10‐day period. A total of 80,000 cells were plated in 24‐well plates followed by EMEM with 10% FBS (positive control) and EMEM plus EMP at 25, 50, 75, and 100 μ g/ml. Cells were harvested on days 1, 3, 5, 7, and 10 and viability was performed utilizing an MTS assay. PDLF proliferation rates were assessed by a CyQUANT GR dye assay. SEM analysis was used to examine the qualitative effects of cellular attachment to diseased root surfaces following EMP compared to nontreated controls. Results: The results indicated that viability was negatively affected for higher doses over time while lower doses displayed viability effects similar to control. Proliferation, however, appeared to be ameliorated following exposure to EMP. The SEM analysis suggests that cellular attachment to diseased dentin was enhanced following EMP application. Conclusion: These in vitro studies support the concept that EMP may act as a suitable matrix for PDLF.