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Observations cliniques, génétiques et microbiologiques d'une famille brésilienne à parodontite agressive
Author(s) -
Trevilatto Paula C.,
Tramontina Vinícius A.,
Machado Maria A. N.,
Gonçalves Reginaldo B.,
Sallum Antonio W.,
Line Sergio R. P.
Publication year - 2002
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2002.290309.x
Subject(s) - treponema denticola , allele , aggressive periodontitis , periodontitis , bacteroides , porphyromonas gingivalis , biology , treponema , prevotella intermedia , proband , genetics , microbiology and biotechnology , immunology , medicine , gene , mutation , bacteria , syphilis , human immunodeficiency virus (hiv)
Background, aim: Aggressive periodontitis comprises a group of rapidly progressive forms of periodontitis. Besides bacteria, a high level of subject susceptibility must be involved in the expression of disease. In the present study, we report the clinical, microbiological and genetic profile of a 14‐individual family with aggressive periodontitis. Method: PCR was utilized to detect pathogenic bacteria of affected sites. DNA was obtained from epithelial cells through a mouthwash with 3% glucose and scrapping of the oral mucosa. RFLP‐PCR was used to analyze cytokine genetic polymorphisms. Results: Localized aggressive periodontitis was diagnosed for an 18‐year‐old systemically healthy non‐smoking proband, with siblings displaying aggressive periodontitis. Bacteroides forsythus and Treponema denticola were the most frequent pathogens. The proband presented Actinobacillus actinomycetemcomitans and detectable levels of Porphyromonas gingivalis , Bacteroides forsythus and Treponema denticola. Allele 2 of IL‐1α (−889) polymorphism was found in all individuals as well as allele 1 of the IL‐1β (+3953) gene. Alleles 1 and 2 (50 % each) of IL‐1β (−511), allele 1 of TNF‐α (−308) and allele 2 (in homo or heterozygosity) of IL‐RN (intron 2) gene were present. Conclusion: The results show that the present microbiological and genetic parameters were not relevant for the prediction of periodontitis susceptibility in this family.