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Modifications de la microflore sous‐gingivale et de la réponse immunitaire humorale après traitement parodontal
Author(s) -
Darby I. B.,
Mooney J.,
Kinane D. F.
Publication year - 2001
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2001.280812.x
Subject(s) - porphyromonas gingivalis , avidity , immune system , treponema denticola , antibody , immunology , periodontitis , prevotella intermedia , microbiology and biotechnology , humoral immunity , chronic periodontitis , periodontal pathogen , biology , medicine
Objectives: To investigate the effect of scaling and root planing (SRP) on the microflora and humoral immune response in adult periodontitis. Materials & Methods: Clinical measurements, subgingival plaque samples, gingival crevicular fluid and sera were taken from 4 sites in 28 adult periodontitis patients before and after SRP. Polymerase chain reaction was used to determine the presence of A. actinomycetemcomitans , P. gingivalis, B. forsythus , P. intermedia , and T. denticola. ELISA was used to investigate the systemic and local antibody titres to these organisms, and thiocyanate dissociation for the determination of serum antibody avidity. Results: SRP produced a good clinical improvement. On a subject basis there was little significant change in the microflora. However, on a site basis, there were significant reductions in P. intermedia , B. forsythus and T. denticola. There was little change in systemic and local antibody titres following SRP, although there was a significant reduction in antibody avidity to P. gingivalis and P. intermedia Conclusion: Post‐therapy clinical improvement was associated with a reduction in bacterial prevalence, but statistical significance was only reached at a site level and this microbial reduction was not significant for all organisms. No significant post‐therapy effects on the humoral immune response were noted other than a reduced antibody avidity to P. gingivalis and P. intermedia. The lack of a clear pattern in the humoral immune response may reflect a failure of the host response to produce adequate levels of biologically functional antibodies, and complex interactions between the subgingival flora and the host response.