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Effets des sels métalliques sur la production orale de composés volatiles sulfurés (VSC)
Author(s) -
Young A.,
Jonski G.,
Rölla G.,
Wåler S. M.
Publication year - 2001
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2001.280809.x
Subject(s) - chemistry , sulfur , saliva , metal , sn2 reaction , cysteine , chromatography , gas chromatography , in vitro , nuclear chemistry , biochemistry , stereochemistry , organic chemistry , enzyme
Background, aim: Halitosis, mainly caused by bacteria located on the posterior dorsum of the tongue and in periodontal pockets, is due to formation of volatile sulfur compounds (VSC). The hypothesis to be tested was that the affinity of a metal for sulfur determines its anti‐VSC activity. Method: Clinical tests were carried out on 12 subjects who rinsed with cysteine to induce halitosis (baseline) before rinsing with 7.34 mM ZnCl 2 , SnF 2 and CuCl 2 . Mouth air VSC analyses were repeated following cysteine rinses at 1 h, 2 h and 3 h using a gas chromatograph. In vitro experiments tested toxic metals Hg 2+ , Pb 2+ and Cd 2+ . 10‐μl aliquots of metal salts were added to 1‐ml aliquots of human whole saliva from 30 subjects. Samples were incubated overnight at 37 o C and saliva headspace was analyzed for VSC in a gas chromatograph. Clinical results: Cu 2+ >Sn 2+ >Zn 2+ (supports hypothesis). Zn 2+ had significantly less anti‐VSC effect compared with Cu 2+ and Sn 2+ at 1, 2 and 3 h. In vitro results indicated that Hg 2+ , Cu 2+ and Cd 2+ had close to 100% anti‐VSC effect, and that Pb 2+ was less effective and Cd 2+ more effective than expected in inhibiting VSC. Conclusions: Apart from Hg 2+ and Cu 2+ , the metals had a significantly greater effect on H 2 S than on CH 3 SH. Cu 2+ and Hg 2+ have well‐known antibacterial activity and may presumably also operate by this mechanism.