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Expression de CD9 et de HLA‐DR par les cellules épithéliales créviculaires et les polymorphonucléaires neutrophiles lors des maladies parodontales
Author(s) -
BissonBoutelliez C.,
Miller N.,
Demarch D.,
Bene M. C.
Publication year - 2001
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2001.028007650.x
Subject(s) - gingivitis , periodontitis , hla dr , medicine , inflammation , immunofluorescence , periodontal disease , immunology , chronic periodontitis , downregulation and upregulation , pathology , antigen , biology , dentistry , antibody , gene , biochemistry
Background, aims: The composition of gingival crevicular fluid (GCF) is likely to reflect inflammatory modifications that take place in the gingiva during periodontal diseases. Method: In this study, GCF was collected at 3 different sites from 23 periodontal patients. The sites were assessed to be healthy, presenting gingivitis or periodontitis. 10 healthy individuals without any form of periodontal disease formed the control group and were sampled at one site each. The cell content of GCF was collected using Durapore Millipore strips, and 2 types of cells were studied: epithelial cells (EC) and polymorphonuclear neutrophils (PMN). The expression of CD9 and HLA‐DR within or on the surface of these cells was studied in immunofluorescence on cytospin smears. Results: Both CD9 and HLA‐DR expression on EC differed significantly from control subjects, and the latter decreased according to the severity of the pathology. None of the PMN found in controls expressed CD9 or HLA‐DR. However, in periodontal patients, the expression of HLA‐DR within PMNs was detectable and increased according to the severity of lesions. CD9 expression on PMNs also increased with inflammation. Conclusion: This study shows that clinically healthy sites of periodontal patients already present signs of immunological activation characterised by a down modulation of HLA‐DR expression on EC and an upregulation of these 2 molecules in PMN.