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Détection par PCR de 5 pathogènes parodontaux putatifs dans des échantillons de plaque dentaire provenant d’enfants de 2 à 12 ans
Author(s) -
Okada Mitsugi,
Hayashi Fumiko,
Nagasaka Nobuo
Publication year - 2001
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2001.028006576.x
Subject(s) - treponema denticola , prevotella intermedia , gingivitis , dental plaque , toothbrush , medicine , periodontitis , dentistry , bacteroides , microbiology and biotechnology , biology , porphyromonas gingivalis , bacteria , brush , electrical engineering , engineering , genetics
Background, aims: The purpose of this study was to detect the presence of Prevotella intermedia , P. nigrescens , Bacteroides forsythus , Treponema denticola , and Campylobacter rectus in plaque samples from 119 children, collected from their toothbrushes using a polymerase chain reaction (PCR). Method: The subjects were 24, 83, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2–12 years old. Plaque samples were collected from all erupted teeth sites using a sterile toothbrush. The mean concentration of DNA recovered from the plaque samples was approximately 640 μg/ml, which was deemed sufficient for performing a PCR‐based survey. Results: The prevalence by PCR in healthy, gingivitis, and periodontitis subjects was 0.0%, 6.0% and 25.0% for P. intermedia , 45.8%, 79.5% and 50.0% for P. nigrescens , 33.3%, 63.9% and 58.3% for B. forsythus , 0.0%, 18.1% and 16.7% for T. denticola , and 100% in duplicate for C. rectus , respectively. Conclusion: Our survey indicated that P. intermedia and T. denticola were more associated with periodontal diseases, B. forsythus and P. nigrescens had a moderate prevalence in all clinical groups, while C. rectus were the most commonly detected species in the oral cavities of children suggesting establishment in their early years.