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Detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in dental plaque samples from children 2 to 12 years of age
Author(s) -
Okada Mitsugi,
Hayashi Fumiko,
Nagasaka Nobuo
Publication year - 2000
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2000.027010763.x
Subject(s) - actinobacillus , gingivitis , porphyromonas gingivalis , toothbrush , medicine , dental plaque , dentistry , periodontitis , brush , electrical engineering , engineering
The purpose of this study was to detect the presence of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in plaque samples from 104 children, collected from their toothbrushes using a polymerase chain reaction (PCR). The age range of all subjects was 2–12 years. 21, 73 and 10 children with healthy gingiva, gingivitis and periodontitis respectively were selected. Plaque samples were collected from all erupted teeth sites using a sterile toothbrush. The mean concentration of DNA recovered from brushing plaque samples was approximately 660 μg/ml, which was sufficient for performing a PCR‐based survey. Both A. actinomycetemcomitans and P. gingivalis were detected in the primary and mixed dentition. The prevalence of A. actinomycetemcomitans in healthy subjects was 4.8%, and those with gingivitis and periodontitis was 6.8% and 20.0% respectively, while the prevalence of P. gingivalis was 4.8% in healthy subjects, and 9.6% and 20.0% in those with gingivitis and periodontitis, respectively. Our survey, using a toothbrush, indicated that A. actinomycetemcomitans and P. gingivalis are rarely present in oral cavities of healthy children.