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Changes in substance P and neurokinin A in gingival crevicular fluid in response to periodontal treatment
Author(s) -
Lundy Fionnuala T.,
Mullally Brian H.,
Burden Donald J.,
Lamey PhilipJohn,
Shaw Christopher,
Linden Gerard J.
Publication year - 2000
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2000.027007526.x
Subject(s) - medicine , substance p , periodontitis , dentistry , clinical attachment loss , neurokinin a , scaling and root planing , periodontal disease , gastroenterology , chronic periodontitis , neuropeptide , receptor
Background: The study of periodontitis provides a unique model for assessing the involvement of neuropeptides in inflammatory disease. Aim: To investigate the effects of periodontal treatment, resulting in a return to periodontal health, on the levels of substance P (SP) and neurokinin A (NKA) in gingival crevicular fluid (GCF). Method: We completed a cause of non‐surgical treatment for 8 subjects with periodontitis (6 females 2 males, mean age 45.1, range 38–67 years) started a course of non‐surgical periodontal treatment. Clinical indices were measured at 2 periodontitis sites at the initial visit and at 8 weeks after the completion of treatment in each subject. A 30‐s sample of GCF was collected from each test site using perio paper strips. Each strip was placed into 500 μl of ice cold 0.1M PBS, pH 7.4, vortex mixed for 30 s, and then stored at −70°C until analysed by radioimmunoassay. Results: The clinical condition of all test sites improved as a result of the periodontal treatment. The levels (pg/30 s sample) of SP fell from 56.3 (SD 66.0) at the initial visit to 4.2 (3.1) after treatment, p =0.017. The concentration (pg/μl) of SP in GCF fell from 140.6 (175.6) to 24.2 (11.1), p =0.036. The levels of NKA fell from 30.5 (17.1) to 10.6 (4.9), p =0.012 whereas the concentration changed little from 85.4 (43.5) to 61.6 (15.1), p =0.41. Conclusion: The reduction in inflammation resulting from effective periodontal treatment is associated with a reduction in the levels of tachykinins in gingival crevicular fluid.