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Methodological considerations in the assessment of gingival crevicular fluid volume
Author(s) -
Deinzer Renate,
Mossanen Babak Saiidi,
Herforth Armin
Publication year - 2000
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2000.027007481.x
Subject(s) - circadian rhythm , reliability (semiconductor) , dentistry , repeated measures design , analysis of variance , orthodontics , medicine , mathematics , statistics , physics , power (physics) , quantum mechanics
In 4 studies on gingival crevicular fluid volume (GCFV) determination, the reliability of measurements, influences of plaque, circadian rhythms and stability over 24 h were examined. Samples were taken at 2 sites with a modified intracrevicular method. Reliability ( n =40): repeated GCFV determinations within 5 min revealed good reliablity coefficients ( r tt >0.80). Influences of supragingival plaque ( n =80): repeated GCFV determinations within 5 min with plaque removal between measurements in fourty subjects, the other subjects serving as control, revealed no group differences with respect to the differences between measurements. Circadian rhythms ( n =20): GCFV was assessed 6× throughout the day. Repeated measures ANOVA revealed no significant time effect. Stability over 24 h under constant clinical conditions ( n =20): measures were taken at 16:00 h on 2 consecutive days, several disturbing variables were kept constant. Retest correlations revealed a low stability (i.e., high variablity) of GCFV measures under constant clinical conditions ( r tt =0.38 for tooth 11 and r tt =−0.25 for tooth 26). It is concluded that GCFV determination can be done with high reliability, the validity of measurements neither being affected by supragingival plaque nor by diurnal rhythms. The low stability of measurements questions the validity of GCFV for diagnostic purposes.