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Lack of antimicrobial effect on periodontopathic bacteria by ultrasonic and sonic scalers in vitro
Author(s) -
Schenk Günther,
Flemmig Thomas F.,
Lob Sibylle,
Ruckdeschel Gotthard,
Hickel Reinhard
Publication year - 2000
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2000.027002116.x
Subject(s) - actinobacillus , porphyromonas gingivalis , microbiology and biotechnology , sonication , ultrasonic sensor , peptostreptococcus , agar , chemistry , bacteria , medicine , biology , anaerobic bacteria , periodontitis , dentistry , chromatography , genetics , radiology
Background: The purpose of this study was to assess the antimicrobial effects of a sonic and ultrasonic scaler generally used for subgingival scaling on gram‐negative and gram‐positive periodontopathic bacteria. Method: Actinobacillus actinomycetemcomitans , Porphyromonas gingivalis , Campylobacter rectus , or Peptostreptococcus micros were suspended in Schaedler's broth medium and treated by a sonic or a magnetostrictive ultrasonic scaler for 30 s and 150 s in vitro. Bacterial suspensions treated by an ultrasonic cell disruptor served as a positive control and untreated bacterial suspensions served as a negative control. Following sonication, samples were serially diluted, streaked on blood agar plates and incubated for 2–5 days at 37°C. Results: Treatment by the sonic or ultrasonic scaler for up to 150 s did not reduce the viability of any of the tested periodontal pathogens. Compared to untreated controls, the viability of A. actinomycetemcomitans and P. gingivalis was significantly ( p <0.05) reduced only following ultrasonication with the cell disruptor after 30 s (0.72 and 0.54 log CFU/ml, respectively) and of A. actinomycetemcomitans , P. gingivalis , C. rectus , and P. micros after 150 s (1.98, 1.34, 1.95 and 1.98 log CFU/ml, respectively). Conclusion: The data of the study may indicate that the assessed sonic and ultrasonic scaler used for subgingival debridement do not result in killing of the tested periodontal pathogens.