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Leukocyte functions in 2 cases of Papillon‐Lefèvre syndrome
Author(s) -
Liu Rongkun,
Cao Caifang,
Meng Huanxing,
Tang Zhihui
Publication year - 2000
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.2000.027001069.x
Subject(s) - chemotaxis , elastase , pathogenesis , lysozyme , granulocyte , immunology , interleukin 8 , medicine , peripheral blood , chemistry , inflammation , enzyme , biochemistry , receptor
Aim: To investigate the rôle of leukocytes in the pathogenesis of Papillon‐Lefèvre syndrome (PLS). Methods: Peripheral blood polymorphonuclear neutrophils (PMNs), monocytes (MNs) and gingival crevicular fluid (GCF) were obtained from 2 cases of PLS with typical features. The chemotaxis of PMNs and MNs were evaluated using a modified Boyden chamber. The adherence of PMNs was determined by adherence of PMNs to petri dishes. Interleukin‐8 (IL‐8) in GCF was detected by sandwich ELISA. Elastase activity in GCF was measured with a low molecular weight substrate (S‐2484) specific for granulocyte elastase. Results: PMNs from both patients showed depressed chemotactic response to FMLP and IL‐8. Total amounts of IL‐8 in GCF from the 2 patients were much higher than those of the normal controls. Elastase activity was not significantly different from that of the controls. The adherence of PMN and the chemotaxis of MN in the 2 patients were normal. Conclusion: The depressed chemotactic response of PMN leads to decreased recruitment of PMN and/or release of lysozyme from PMN in the diseased gingival tissue, increasing the susceptibility of PLS patients to periodontal infection.