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Intra‐familial distribution of Fusobacterium nucleatumstrains in healthy families with optimal plaque control
Author(s) -
SuchettKaye G.,
Décoret D.,
Barsotti O.
Publication year - 1999
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.1999.260611.x
Subject(s) - fusobacterium nucleatum , restriction enzyme , dna , ethidium bromide , biology , microbiology and biotechnology , porphyromonas gingivalis , genetics , bacteria
. Fusobacterium nucleatum , a Gram‐negative anaerobic rod associated with periodontal disease, is also found in healthy individuals and is considered part of the indigenous oral microflora. Although intra‐familial transmission of periodontal pathogens has been documented, there are no data relating transmission of F. nucleatum . This study investigated the distribution of F. nucleatum strains in 4 strictly healthy families. 32 F. nucleatum strains were isolated from 19 individuals (8 parents and 11 children aged 1–13 years). DNA was extracted and digested with the restriction endonucleases Eco RI, Taq I and Hind III. The digests were separated by electrophoresis through 0.8% agarose gels at 40 V overnight, in TBE buffer containing 1 μg/ml ethidium bromide, and photographed. The DNA was transferred to nylon filters by Southern blotting and hybridized with a digoxigenin labelled E. coli rRNA probe (Kit Dig DNA Labelling mixture – Boehringer). Probed DNA was visualized colorimetrically (CSPD Luminescent Detection Kit Boehringer) and photographed (Amersham). We found that 10/11 children shared identical ribotypes with at least one of their respective parents. Some of the children also harbored a unique additional ribotype. On the basis of indistinguishable restriction endonuclease and ribotype patterns these results support the hypothesis that intra‐familial transmission of F. nucleatum is possible.