Premium
Antibiotic susceptibility of putative periodontal pathogens in advanced periodontitis patients
Author(s) -
Kleinfelder J. W.,
Müller R. F.,
Lange D. E.
Publication year - 1999
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.1999.260603.x
Subject(s) - fusobacterium nucleatum , microbiology and biotechnology , prevotella intermedia , porphyromonas gingivalis , antibiotics , amoxicillin , periodontitis , peptostreptococcus , medicine , fusobacterium , metronidazole , tetracycline , antibiotic resistance , biology , anaerobic bacteria , dentistry , bacteroides , bacteria , genetics
. In the present study, the antibiotic susceptibility of most prevalent micro‐organisms in advanced periodontitis patients was evaluated. In 56 patients, pooled subgingival plaque samples were taken from the deepest site of each quadrant and were cultivated anaerobically. From each patient, the 4 most frequently encountered types of bacterial colonies were subcultured and identified (Rapid ID 32 A). From all bacterial species identified in the 224 subcultures, the 4 most prevalent were used for susceptibility testing to tetracycline, metronidazole and amoxicillin/clavulanate using the E Test. The most prevalent microorganisms were Fusobacterium nucleatum (38/214), Peptostreptococcus micros (33/214), Prevotella oralis (33/214) and Porphyromonas gingivalis (32/214). Regarding antibiotic susceptibility it could be shown that minimal inhibitory concentration (MIC) in all cases was below antibiotic concentrations achievable in gingival crevicular fluid. However, antibiotic resistance was seen in 3 to 29% of the investigated microorganisms.