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Metronidazole susceptibility testing of anaerobic bacteria associated with periodontal disease
Author(s) -
Poulet P. P.,
Duffaut D.,
Lodter J. Ph.
Publication year - 1999
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.1999.260411.x
Subject(s) - metronidazole , anaerobic bacteria , periodontal disease , microbiology and biotechnology , anaerobic exercise , medicine , bacteria , dentistry , biology , antibiotics , genetics , physiology
. The aim of the present investigation was to determine the susceptibility of Prevotella intermedia , Porphyromonas gingivalis , Fusobacterium and Peptostreptococcus micros to metronidazole in vitro. Two methods were applied on each isolated strain: agar dilution and epsilometer Etest®. A total of fifty three wild test strains (13 P.intermedia , 14 P.gingivalis , 14 F.spp and 12 P.micros ) were isolated from patients with periodontitis. The Etest® appears to be a simple, rapid and reliable method for the metronidazole susceptibility testing. The results show that all P.intermedia , P.gingivalis and F.spp strains were susceptible to metronidazole. The mean values of minimal inhibitory concentration obtained with the agar dilution method were, respectively, 0.98 μg/ml, 0.122 μg/ml and 0.242 μg/ml. For P. micros , the minimal inhibitory concentration was of 12.14 μg/ml. Comparatively to break points, only 60% of P.micros strains seem to be susceptible, in vitro, to metronidazole. This study demonstrated the excellent activity of metronidazole against P.intermedia , P.gingivalis , F.spp except perhaps for P.micros .