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Effects of nicotine on periodontal ligament fibroblasts in vitro
Author(s) -
Giannopoulou Catherine,
Geinoz Anne,
Cimasoni Giorgio
Publication year - 1999
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1034/j.1600-051x.1999.260109.x
Subject(s) - periodontal fiber , nicotine , chemotaxis , alkaline phosphatase , in vitro , chemistry , incubation , periodontitis , phosphatase , andrology , pharmacology , biochemistry , medicine , dentistry , biology , enzyme , receptor
. Cigarette smoking is associated with increased incidence of periodontal disease and poor response to therapy. In the present study, we examined the effects of nicotine on several functions of periodontal ligament fibroblasts (PDLF): proliferation, attachment, alkaline phosphatase production and chemotaxis. Nicotine concentrations varying from 5 ng/ml to 250 μg7sol;ml were tested. Proliferation of cells was studied by the incorporation of 3H‐thymidine, and a dose‐dependent inhibition was observed with concentrations ≥100 ng/ml. Similar results were observed when studying the attachment of the cells on plastic surfaces, using a colorimetric method. The inhibition of attachment was even more evident after 6 h incubation of the cells with nicotine. The activity of alkaline phosphatase, as determined with the substrate p‐nitrophenyl phosphate, in both conditioned medium (CM) and cellular extract (CE), was also significantly decreased in a concentration‐related fashion. Finally, the chemotaxis of PDLF, as examined by a modification of the Boyden's blind‐well chamber technique, was inhibited in a dose‐dependent manner. The degree of inhibition varied from 15% with the lowest concentration of nicotine (50 ng/ml), to almost 90% with the highest (5 μg/ml). The results show that nicotine can have direct adverse effects on various functions of the periodontal cells.