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Production of transforming growth factor β 1 and prostaglandin E 2 by osteoblast‐like cells cultured on titanium surfaces blasted with TiO 2 particles
Author(s) -
Mustafa Kamal,
Rubinstein Joanna,
Lopez Blanca Silva,
Arvidson Kristina
Publication year - 2003
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1034/j.1600-0501.2003.140107.x
Subject(s) - osteoblast , titanium , growth factor , radioimmunoassay , transforming growth factor , surface roughness , chemistry , prostaglandin e2 , bone cell , prostaglandin e , scanning electron microscope , cell growth , materials science , endocrinology , in vitro , biochemistry , biology , metallurgy , composite material , receptor
The surface roughness of an implant to which osteoblasts attach may influence endogenous expression of growth factor and cytokines at the implant–tissue interface, modulating the healing process and affecting the quality of bone formation. The present study, using cells derived from human mandibular bone, investigated the effect of varying roughness of titanium surfaces on production of transforming growth factor β 1 (TGF‐ β 1 ) and prostaglandin E 2 (PGE 2 ). The titanium surfaces were turned (control) and then roughened by blasting with 63–90 µm, 106–180 µm or 180–300 µm TiO 2 particles. The cells were cultured onto the surfaces till confluence was achieved. Fresh media were added in the presence or absence of 1,25‐dihydroxyvitamin D 3 [1,25‐(OH) 2 D 3 ], the stimulator of osteogenic differentiation, and aliquots of conditioned cell media were used for assay 24 h later. Cellular morphology was determined by scanning electron microscopy. Cellular production of TGF‐ β 1 and PGE 2 on each surface was assessed by enzyme‐linked immunosorbent assay (ELISA) and radioimmunoassay (RIA), respectively, using commercially available kits. All blasted surfaces showed significantly higher production of TGF‐ β 1 than the turned surfaces ( P < 0.05). In response to stimulation by 1,25‐(OH) 2 D 3 cellular production of TGF‐ β 1 , was also significantly greater ( P < 0.05) on the blasted surfaces than on the turned one. The total amount of PGE 2 in the conditioned media was higher than on the turned surfaces in the presence or absence of 1,25‐(OH) 2 D 3 . There were no significant differences among the three blasted surfaces with respect to production of the local factors. However, we could not show a synergistic effect of surface roughness and vitamin D on the production of both TGF‐ β 1 and PGE 2 using primary cell culture model.