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Effects of titanium surfaces blasted with TiO 2 particles on the initial attachment of cells derived from human mandibular bone
Author(s) -
Mustafa Kamal,
Wroblewski Joanna,
Hultenby Kjell,
Silva Lopez Blanca,
Arvidson Kristina
Publication year - 2000
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1034/j.1600-0501.2000.110204.x
Subject(s) - titanium , materials science , scanning electron microscope , morphology (biology) , texture (cosmology) , micrograph , nanotechnology , composite material , metallurgy , genetics , image (mathematics) , artificial intelligence , computer science , biology
This study was performed to determine the effect of commercially pure titanium surfaces blasted with TiO 2 particles on the biological responses of cells derived from human mandibular bone. The morphology and attachment of those cells were investigated on turned titanium surfaces (control) and surfaces blasted with 45 μm (standard), 45–63 μm, and 63–90 μm TiO 2 particles. The surfaces were analyzed in a scanning electron microscope. Based on surface analyses reported elsewhere, the turned samples had the smoothest surfaces and the roughest were those blasted with the largest particles (63–90 μm). The cell profile areas were measured using a semi‐automatic interactive image analyzer. The attachment was determined as a ratio of the area of cell profiles and the total micrograph area and was expressed as percentage of attachment. Morphologically, the cells were heterogeneous. In general, the cells had spread well on all titanium surfaces, indicating good attachment to both smooth and rough surfaces. After 1, 3 and 6 h, the percentage of cell attachment did not differ significantly between the surfaces blasted with 63–90 μm and the turned surfaces, but was significantly lower on the surfaces blasted with 45 μm or 45–63 μm particles. After 24 h the surfaces blasted with 63–90 μm particles had a higher rate of cell attachment than all the other surfaces including the controls. It is concluded that attachment and growth of cells originating from human mandibular bone in vitro , are influenced by the micro‐texture of the implant surface.