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Attachment and proliferation of human oral fibroblasts to titanium surfaces blasted with TiO 2 particles. A scanning electron microscopic and histomorphometric analysis.
Author(s) -
Mustafa Kamal,
Lopez Blanca Silva,
Hultenby Kjeil,
Wennerberg Ann,
Arvidson Kristina
Publication year - 1998
Publication title -
clinical oral implants research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.407
H-Index - 161
eISSN - 1600-0501
pISSN - 0905-7161
DOI - 10.1034/j.1600-0501.1998.090307.x
Subject(s) - titanium , scanning electron microscope , materials science , fibroblast , morphology (biology) , nanotechnology , composite material , biophysics , chemistry , metallurgy , in vitro , biochemistry , biology , genetics
The purpose of this study was to determine the effect of c.p. titanium surfaces blasted with TiO 2 particles on the biological responses of human gingival fibroblasts (HGF). Fibroblast morphology and attachment were investigated on turned (control) titanium surfaces and those blasted with 45 μm (standard), 45Ndash;63 μm, and 63–90 μm TiO 2 particles. The specimens were analyzed using a confocal laser scanner and SEM. The cell profile areas were measured using a semiautomatic interactive image analyser. The figures were expressed as percent of attachment. The turned samples had the smoothest surfaces and the roughest were those blasted with 63–90 μm. All TiO 2 blasted specimens had homogeneous surfaces. Cells appeared to flatten, spread and form cellular bridges with the adjacent cells. Fibroblasts on the turned titanium surfaces appeared to follow the direction of the fine irregularities on the surface but tended to spread haphazardly on the blasted surfaces. The attachment assays showed no significant difference in the percentage of fibroblast cell attachment on the standard surfaces compared to the turned surfaces. Both surfaces blasted with 45–63 μm or 63–90 μm had significantly ( P <0.05) lower percentages of cell attachment than the control. The surfaces blasted with 63–90 μm particles had the lowest rate of cell attachment. A significant correlation ( P <0.01) was found between the degree of particle size and attachment of fibroblasts after l–72 h. It is concluded that surface micro‐texture influences the attachment and growth of HGF: surfaces blasted with 45 pm TiO, do not inhibit fibroblast attachment and smooth or finely grooved surfaces could be conducive to cellular attachment.