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Usefulness of different techniques in the study of the epidemiology of salmonellosis
Author(s) -
RUIZ M.,
RODRÍGUEZ J. C.,
SIRVENT E.,
ESCRIBANO I.,
CEBRIÁN L.,
ROYO G.
Publication year - 2003
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2003.1110903.x
Subject(s) - serotype , typhoid fever , epidemiology , outbreak , salmonella , rapd , biology , microbiology and biotechnology , virology , medicine , environmental health , genetics , bacteria , pathology , population , genetic diversity
Our objective was to evaluate the usefulness of serotyping, phagotyping, antibiotyping and RAPD (random amplified polymorphic DNA) in the study of the epidemiology of salmonellosis in Elche (Spain). We examined 1232 clinical isolates of non‐typhoid Salmonella serotypes. Serotyping adequately differentiates 12.3% of the isolates and phagotyping raises this percentage to 37%. Antibiotyping is a suitable complement in certain circumstances, such as in the case of specific outbreaks. RAPD is a fast and simple technique which is within the reach of most laboratories, complementing the results obtained by antibiotyping and serotyping, in order to be able to control outbreaks of salmonellosis in everyday practice.