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Tumour‐type‐specific capillary endothelial cell stainability in malignant B‐cell lymphomas using antibodies against CD31, CD34 and Factor VIII
Author(s) -
NORRBY KLAS,
RIDELL BÖRJE
Publication year - 2003
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2003.1110406.x
Subject(s) - cd31 , pathology , cd34 , immunohistochemistry , staining , lymphatic system , microvessel , lymph node , antibody , chemistry , biology , medicine , immunology , stem cell , microbiology and biotechnology
The microvessel density (MVD) was assessed in lymph nodes infiltrated by diffuse large B‐cell lymphomas, mantle cell lymphomas, chronic lymphatic leukemia and follicular lymphomas, and in lymphadenitis. Serial sections of formalin‐fixed and paraffin‐embedded tissue were stained with antibodies against CD31, CD34 or Factor VIII. Using light microscopy and computerised image analysis, the number and size of individual immunostained vessel profiles within a preselected area size range corresponding to capillaries, postcapillary venules, small collecting venules and small arterioles were determined. A significantly larger number of vessels were registered following staining with anti‐CD34 than with anti‐CD31 or anti‐Factor VIII. Moreover, among the smallest capillary‐sized vessel profiles in all lesion types, there was a selective relative loss of stainability of anti‐CD31 and anti‐Factor VIII, resulting in a substantial total loss of visualised capillary‐sized vessels compared with anti‐CD34. In fact, the number of non‐detected capillaries following staining with anti‐CD31 and anti‐Factor VIII was significantly tumour type specific. These findings influence how we evaluate MVD data in B‐cell lymphomas and possibly also other tumour types, as well as data relating to capillary endothelium‐related functional variables of proliferation, apoptosis and maturation when different double‐labelling immunohistochemical techniques are used and different tumour types are analysed.