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Postmitotic basal cells in squamous cell epithelia are identified with Dolichos biflorus agglutinin – functional consequences Note
Author(s) -
HRDLIČKOVÁCELA ENKELA,
PLZÁK JAN,
HOLÍKOVÁ ZUZANA,
DVOŘÁNKOVÁ BARBORA,
SMETANA KAREL
Publication year - 2001
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2001.d01-137.x
Subject(s) - epidermis (zoology) , basal (medicine) , biology , lectin , agglutinin , golgi apparatus , microbiology and biotechnology , cellular differentiation , cell , biochemistry , anatomy , gene , endocrinology , insulin
Dolichos biflorus agglutinin (DBA) is a plant lectin specifically recognizing α‐N‐acetylgalactosamine. Controversial reports regarding the binding of DBA to the epidermis have been published. Using a double labeling procedure at the single‐cell level, we studied the expression of DBA‐reactive binding sites in conjunction with markers of cell proliferation and differentiation in normal human epidermis, cornea, and malignant tumors as well as in cultured keratinocytes. The results characterize the cells recognized by DBA as postmitotic early differentiating cells, identifiable by their lack of expression of the proliferation marker (Ki‐67). The Golgi complex of a limited number of cultured keratinocytes was recognized by DBA and some of these cells show the accumulation of β 1 integrin chain in the Golgi complex. This process seems to be important for the migration of postmitotic cells from the basal to the suprabasal layers.