Premium
Quantitative cell and matrix changes in human humeral head osteoarthritic cartilage
Author(s) -
Mori RYUJI,
Ochi MITSUO,
Sakai YASUO,
Maniwa SOKICHI,
Kawasaki KENZO
Publication year - 2000
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1034/j.1600-0463.2000.d01-10.x
Subject(s) - cartilage , osteoarthritis , confocal , pathology , pannus , cell , anatomy , degeneration (medical) , chondrocyte , biomedical engineering , matrix (chemical analysis) , medicine , chemistry , arthritis , chromatography , geometry , alternative medicine , mathematics , biochemistry
A new quantitative method was devised both to establish an objective standard for morphometric diagnosis and to determine the extent of degeneration in osteoarthritic cartilage. Eight normal and forty‐eight osteoarthritic humeral heads, subsequently confirmed by light microscopy, were obtained at necropsy. The articular cartilage was observed in situ with a laser scanning confocal microscope (LSCM) and morphometric measurements determined cell density (cells/mm), cell volume fraction (%) and mean cell volume (μ 3 ). The osteoarthritic cartilages were classified according to the following four characteristics: increase in thickness, increase in cell volume fraction, decrease in cell volume fraction, and fibrous pannus. Deviations in cell density and cell volume fraction from normal means were calculated as extent of degeneration. Our present approach aims to provide valuable clues, such as objective stereological information and a unique reference for biochemical and traditional morphlogical analyses, that clinicians will be able to use in combination with other methods in order to establish a reliable diagnosis.