Open AccessDetection of oncogenic human papillomavirus in sporadic retinoblastoma
Author(s) -
Palazzi M. A.,
Yunes J. A.,
Cardinalli I. A.,
Stangenhaus G. P.,
Brandalise S. R.,
Ferreira S. A.,
Sobrinho J. S. P.,
Villa L. L.
Publication year - 2003
Publication title -
acta ophthalmologica scandinavica
Language(s) - English
Resource type - Journals
eISSN - 1600-0420
pISSN - 1395-3907
DOI - 10.1034/j.1600-0420.2003.00112.x
Subject(s) - retinoblastoma , polymerase chain reaction , medicine , southern blot , human papillomavirus , dot blot , pathology , dna , cancer research , virology , gene , biology , genetics
. Purpose: To investigate the presence of human papillomavirus (HPV) DNA in tumour tissue from patients with unilateral retinoblastoma. Methods: Samples of paraffin‐embedded tumour tissue from 43 children with unilateral retinoblastoma were collected to investigate the presence of HPV DNA using polymerase chain reaction (PCR) and dot blot hybridization. Results: Oncogenic HPV DNA types 16 and 35 were detected in 12 (27.9%) of 43 tumour specimens. A higher frequency of differentiated tumours (63.3%) was observed among the HPV‐positive tumours. Conclusions: Future studies are necessary to demonstrate an association between HPV and sporadic retinoblastoma.