Open AccessA clinical study comparing PureSperm and SpermFilter for density gradient separation of human spermatozoa in assisted reproduction
Author(s) -
Laursen Steen B.,
Andersen Claus Yding,
Hindkjær Johnny,
Erb Karin
Publication year - 2003
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1034/j.1600-0412.2003.00297.x
Subject(s) - semen , intracytoplasmic sperm injection , insemination , sperm washing , artificial insemination , in vitro fertilisation , human fertilization , andrology , assisted reproductive technology , fertility , gynecology , reproduction , sperm , medicine , fertility clinic , intrauterine insemination , biology , pregnancy rate , pregnancy , infertility , population , anatomy , ecology , environmental health , genetics
Objective. To compare a new density gradient medium, SpermFilter, for purifying spermatozoa in assisted reproduction with the more established medium, PureSperm. Design. Part 1, a multicenter study on 225 semen samples purified using either PureSperm (115 semen samples) or SpermFilter (110 semen samples). Part 2, a retrospective, single center study on a total of 898 assisted reproductive cycles (245 insemination cycles using husband semen, 58 insemination cycles using donor semen and 595 in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles. Setting. Part 1, three fertility clinics in Denmark (two university‐affiliated fertility clinics and one private clinic). Part 2, one university‐affiliated fertility clinic in Denmark. Main outcome parameters. Part 1, purity of purified spermatozoa (% motile), motility index and recovery of motile spermatozoa. Part 2, malformation and baby take‐home rates (insemination cycles), fertilization, cleavage, implantation, malformation and baby take‐home rates (IVF/ICSI cycles). Results. No statistical differences were observed in any of the parameters investigated. Conclusion. SpermFilter is a valid alternative to PureSperm in assisted reproduction technology (ART).