Open AccessSoluble tumor necrosis factor receptor II and soluble cell adhesion molecule 1 as markers of tumor necrosis factor‐α release in preeclampsia
Author(s) -
Visser Wil,
Beckmann Ilse,
Knook Marco A. H.,
Wallenburg Henk C. S.
Publication year - 2002
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1034/j.1600-0412.2002.810805.x
Subject(s) - tumor necrosis factor alpha , medicine , necrosis , cell adhesion molecule , receptor , endocrinology , tumor necrosis factor receptor 1 , cell adhesion , pathology , immunology , cell , chemistry , biochemistry , tumor necrosis factor receptor
Background. The purpose of this case‐controlled study was to investigate whether plasma concentrations of TNF‐receptors I and II and tumor necrosis factor‐α‐induced cell adhesion molecule 1 VCAM‐1 could serve as more sensitive markers of tumor necrosis factor‐α release in preeclamptic women than a direct measurement of circulating tumor necrosis factor‐α. Methods. Plasma concentrations of soluble tumor necrosis factor receptor I and II, immunoreactive tumor necrosis factor‐a and soluble cell adhesion molecule VCAM‐1 were determined in 21 patients with severe proteinuric preeclampsia (23–35 weeks' gestation) and 21 gestational age‐matched normotensive controls by enzyme‐linked immunoassays. Concentrations of bioactive tumor necrosis factor‐α were assessed by the WEHI 164 bioassay. Data were statistically evaluated by Wilcoxon's rank sum and sign tests, and Spearman's test was used to evaluate clinical and biochemical correlations. Results. Bioactive tumor necrosis factor‐α was detected in 19 of 21 preeclamptic and 18 of 21 normotensive women, with no difference in plasma concentrations between both groups. Immunoreactive tumor necrosis factor‐α, soluble TNF‐receptors and soluble cell adhesion molecule VCAM‐1 were significantly increased in plasma of preeclamptic patients, and a statistically significant positive correlation was observed between immunoreactive tumor necrosis factor‐α and TNFRII. In preeclamptic patients a statistically significant negative correlation was observed between TNFRII and platelet count, and between soluble cell adhesion molecule VCAM‐1 and birthweight ratio. Conclusion. These results show that plasma concentrations of soluble tumor necrosis factor receptor II and soluble cell adhesion molecule VCAM‐1 reflect the release of tumor necrosis factor‐α and provide sensitive markers of excessive release of this cytokine in preeclampsia.