Open AccessEffect of ceramide analogs on interleukin‐1α‐induced production of prostaglandin E 2 by amnion‐derived (WISH) cells
Author(s) -
Nakamura Satomi,
Kawano Yasushi,
Kamihigashi Shoko,
Sugano Terumasa,
Narahara Hisashi,
Miyakawa Isao
Publication year - 2001
Publication title -
acta obstetricia et gynecologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.401
H-Index - 102
eISSN - 1600-0412
pISSN - 0001-6349
DOI - 10.1034/j.1600-0412.2001.800103.x
Subject(s) - medicine , amnion , ceramide , prostaglandin , interleukin , prostaglandin e2 , wish , interleukin 22 , cytokine , endocrinology , pregnancy , biochemistry , biology , fetus , genetics , apoptosis , sociology , anthropology
Background. Prostaglandin E 2 (PGE 2 ) is reportedly synthesized in the amnion, and its levels are increased during labor. Our objective was to measure the level of PGE 2 induced by interleukin (IL)‐1α following treatment with ceramide analogs in amnion‐derived cells. Methods. Amnion‐derived (WISH) cells were cultured and stimulated by IL‐1α, IL‐1 receptor antagonist (ra), C2‐ceramide and C6‐ceramide. The levels of PGE 2 in the media were measured by ELISA. The induction of prostaglandin H synthase (PGHS)‐2mRNA was detected by reverse transcription and polymerase chain reaction (RT‐PCR). Results. Following stimulation with IL‐1α, the production of PGE 2 could not be detected until incubation had continued for 2 h, but this production appeared to continue after 4 h of incubation. The production of PGE 2 was significantly increased by IL‐1α, and was suppressed by IL‐1 ra, in a dose‐dependent manner. PGE 2 production was significantly increased by IL‐1α and C2‐ceramide as compared with IL‐1α alone. However, PGE 2 production was not significantly increased by IL‐1α and C6‐ceramide as compared with IL‐1α alone. PGHS‐2 mRNA were induced by treatment with IL‐1α, and were strongly induced by treatment with IL‐1α and C2‐ceramide by RT‐PCR. Conclusions. Results suggest that IL‐1α induce the PGHS‐2 mRNA and stimulate the production of PGE 2 by a mechanism that involves the sphingomyelin‐ceramide system. Ceramide may be important in increasing the production of PGE 2 during parturition.