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Analysis of effector CD4 (OX‐40 + ) and CD8 (CD45RA + CD27 ‐ ) T lymphocytes in active multiple sclerosis
Author(s) -
Hintzen R. Q.,
Pot K.,
Paty D.,
Oger J.
Publication year - 2000
Publication title -
acta neurologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.967
H-Index - 95
eISSN - 1600-0404
pISSN - 0001-6314
DOI - 10.1034/j.1600-0404.2000.00007.x
Subject(s) - effector , cd8 , immunology , cytotoxic t cell , biology , multiple sclerosis , t lymphocyte , lymphocyte , microbiology and biotechnology , myelin , in vitro , antigen , endocrinology , central nervous system , biochemistry
Objectives ‐ Recently, effector T‐cell subpopulations have been identified that can be distinguished by expression of members of the TNF‐R family: CD4 + OX‐40 + cells are CD4 helper‐effector cells CD8 + CD45RA + CD27 ‐ cells are CD8‐killer‐effector cells. We investigatedwhether these lymphocyte subsets were increased in the active phase of multiple sclerosis (MS). Material and methods ‐ Multiple colourimmunofluorescence staining was performed on peripheral blood lymphocytes of 28 patients with active MS and of 29 healthy controls,followed by FACS analysis. Results ‐ Frequencies of CD8‐killer‐effector cells showed a wide interindividual range in both groups and percentagesof CD4 helper‐effector cells were low. No significant difference between the groups was observed for these subsets, but CD8 + CD45RA ‐ CD27 ‐ were increased in MS. In healthy individuals, CD4 helper‐effector cells correlated with the total percentages of memory cells. Moreover, CD4 + and CD8 + memory cells were strongly correlated. Conclusions ‐ The here described recently identified effector CD4 and CD8 lymphocyte subpopulations were not increased in clinically active MS. It is however still possible that in MS, myelin‐specific encephalitogenic cells reside within these subsets.