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Effects of intravenous anesthetics on interleukin (IL)‐6 and IL‐10 production by lipopolysaccharide‐stimulated mononuclear cells from healthy volunteers
Author(s) -
Takaono M.,
Yogosawa T.,
OkawaTakatsuji M.,
Aotsuka S.
Publication year - 2002
Publication title -
acta anaesthesiologica scandinavica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.738
H-Index - 107
eISSN - 1399-6576
pISSN - 0001-5172
DOI - 10.1034/j.1399-6576.2002.460209.x
Subject(s) - proinflammatory cytokine , medicine , lipopolysaccharide , peripheral blood mononuclear cell , cytokine , propofol , pharmacology , midazolam , interleukin , immunology , in vitro , inflammation , biology , biochemistry , sedation
Background: Surgical trauma has been shown to augment the plasma concentrations of proinflammatory cytokines, which are important mediators of host defense mechanisms and the systemic inflammatory response syndrome (SIRS). Recently, it has been shown that certain kinds of surgery provoke not only a proinflammatory response (SIRS) but also a concurrent anti‐inflammatory response. The aim of this study was therefore to examine the effects of intravenous anesthetics on the synthesis of interleukin (IL)‐6 (a proinflammatory cytokine) and IL‐10 (an anti‐inflammatory cytokine) by lipopolysaccharide (LPS)‐stimulated mononuclear cells from healthy volunteers. Methods: Peripheral blood mononuclear cells (PBMCs) from 17 healthy volunteers, separated by centrifugation on a Ficoll‐Hypaque gradient, were washed and suspended in RPMI containing 10% heat‐inactivated fetal calf serum (FCS). After adding RPMI‐FCS containing various concentrations of intravenous anesthetics (propofol, thiopental, ketamine and midazolam), the PBMCs were incubated overnight in the presence of a submaximal concentration of LPS. The supernatants were collected and their IL‐6 and IL‐10 contents were assayed using enzyme‐linked immunosorbent assay kits. Results: Propofol inhibited both IL‐6 and IL‐10 production at 0.5 µg/mL, 5 µg/mL and 50 µg/mL. Conversely, thiopental induced IL‐10 production at 2 µg/mL and 20 µg/mL. Conclusion: Propofol appears to inhibit both IL‐6 and IL‐10 production by LPS‐stimulated PBMCs in vitro . Further study is required to clarify the mechanism of the suppressive effect of propofol.