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3′(2′)‐Phosphoadenosine 5′‐phosphate phosphatase is reduced in postmortem frontal cortex of bipolar patients
Author(s) -
Shaltiel G,
Kozlovsky N,
Belmaker R H,
Agam G
Publication year - 2002
Publication title -
bipolar disorders
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.285
H-Index - 129
eISSN - 1399-5618
pISSN - 1398-5647
DOI - 10.1034/j.1399-5618.2002.01197.x
Subject(s) - bipolar disorder , phosphatase , enzyme , western blot , etiology , medicine , alkaline phosphatase , lithium (medication) , chemistry , psychology , endocrinology , biology , microbiology and biotechnology , biochemistry , gene
Shaltiel G, Kozlovsky N, Belmaker RH, Agam G. 3′(2′)‐Phosphoadenosine 5′‐phosphate phosphatase is reduced in postmortem frontal cortex of bipolar patients. Bipolar Disord 2002: 4: 302–306. © Blackwell Munksgaard, 2002Objective:3′(2′)‐Phosphoadenosine 5′‐phosphate (PAP) phosphatase is a novel lithium (Li) inhibitable enzyme. Thus the enzyme seemed an important candidate for studies of the molecular etiology of bipolar disorder.Methods:RT–PCR, Western‐blot analysis and Pi liberation were used to measure PAP phosphatase mRNA levels, protein levels and enzyme activity (respectively) in postmortem frontal cortex specimens of bipolar patients versus normal subjects.Results:The PAP phosphatase protein levels were 24% significantly lower in bipolar patients than in normal subjects. PAP phosphatase mRNA levels and enzymatic activity did not differ between normal controls and bipolar patients.Conclusions:Abnormality of PAP phosphatase in bipolar patients offers a new direction for study of bipolar disorder etiology.