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HLA class II genetic association of type 1 diabetes mellitus in Czech children
Author(s) -
Cinek Ondrej,
Koloušková Stanislava,
Šnajderová Marta,
Šumník Zdeněk,
Sedláková P,
Dřevínek Pavel,
Vavřinec Jan,
Ronningen Kjersti S
Publication year - 2001
Publication title -
pediatric diabetes
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.678
H-Index - 75
eISSN - 1399-5448
pISSN - 1399-543X
DOI - 10.1034/j.1399-5448.2001.002003098.x
Subject(s) - hla dqb1 , genotyping , human leukocyte antigen , allele , medicine , odds ratio , type 1 diabetes , haplotype , diabetes mellitus , genotype , subtyping , hla dq , polymerase chain reaction , genetics , immunology , biology , antigen , endocrinology , gene , computer science , programming language
Abstract: To examine human leukocyte antigen (HLA) class II association of type 1 diabetes mellitus (DM) in Czech children, we performed a case–control study of 261 patients diagnosed before the age of 15 and 289 non‐diabetic control children. Complete HLA‐DQA1, DQB1 genotyping and DRB1*04 subtyping were carried out by polymerase chain reactions with sequence‐specific primers. The effect of the DRB1*04 subtypes was studied in DRB1*04 alleles carried on DQB1*0302‐DQA1*03 haplotypes. The risk was statistically evaluated by testing 2 × 2 tables, considering corrected p‐values < 0.05 significant. The DQB1*0302 (odds ratio, OR = 9.0), DQB1*0201 (OR = 3.4) and DQA1*03 (OR = 7.5) alleles were significantly associated with diabetes risk, while the DQB1*0602 (OR = 0.02), DQB1*0301 (OR = 0.08), DQB1*0503 (OR = 0.13), DQB1*0603 (OR = 0.20), DQA1*01 (OR = 0.28) and DQA1*02 (OR = 0.26) alleles were significantly protective. Of the DQA1‐DQB1 genotypes, we point out the extremely high risk of OR = 116 conferred by HLA‐DQA1*05‐DQB1*0201/DQA1*03‐DQB1*0302. Among DRB1*04 subtypes, DRB1*0403 was significantly protective (OR = 0.05, CI 95% 0.01–0.45). Since none of the remaining DRB1*04 subtypes was associated with type 1 DM, our study may present another piece of evidence that the DRB1*0401 and DRB1*0404 alleles do not modify type 1 diabetes risk generally in European populations.