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Detection of porcine endogenous retrovirus in cultures of freshly isolated porcine bone marrow cells
Author(s) -
McIntyre Matiza C.,
Kannan Bhanumathi,
SolanoAguilar Gloria I.,
Wilson Carolyn A.,
Bloom Eda T.
Publication year - 2003
Publication title -
xenotransplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.052
H-Index - 61
eISSN - 1399-3089
pISSN - 0908-665X
DOI - 10.1034/j.1399-3089.2003.02044.x
Subject(s) - xenotransplantation , bone marrow , biology , haematopoiesis , reverse transcriptase , virology , endogenous retrovirus , retrovirus , cell culture , immunology , microbiology and biotechnology , stem cell , transplantation , virus , rna , medicine , gene , biochemistry , genetics , genome
Pigs are under consideration as possible sources of organs for xenotransplantation in humans. The induction of hematopoietic microchimerism through xenotransplantation of source animal hematopoietic cells has been suggested as a means to induce tolerance in potential recipients. Because all porcine cells contain genetic information for porcine endogenous retrovirus (PERV), coculture techniques, reverse transcriptase (RT) and reverse transcriptase‐polymerase chain reaction assays were used to determine whether infectious PERV is released from fresh porcine bone marrow cells cultured in the presence or absence of porcine cytokines. Human embryonic kidney cell line, HEK‐293 cells cocultured with porcine bone marrow cells were positive for PERV RNA but never became positive for viral RT activity, suggesting the PERV infection was not productive. In contrast, high levels of RT activity was detected in porcine ST‐IOWA cells after coculture, demonstrating that these cells became productively infected. PERV was released from cultured porcine bone marrow cells without stimulation, and combinations of the porcine hematopoietic cytokines, interleukin‐3, granulocyte macrophage‐colony stimulating factor and stem cell factor had no additional effect on the infectivity or in vitro tropism of released PERV virions.