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Expression of defender against apoptotic death ( DAD‐1 ) in Iris and Dianthus petals
Author(s) -
Kop Dianne A.M. van der,
Ruys Gerda,
Dees Dianka,
Van Der Schoot Chris,
Douwe de Boer A.,
Van Doorn Wouter G.
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2003.1170213.x
Subject(s) - petal , carnation , dianthus , programmed cell death , biology , apoptosis , microbiology and biotechnology , epidermis (zoology) , iris (biosensor) , botany , genetics , anatomy , computer security , computer science , biometrics
The gene defender against apoptotic death ( DAD‐1 ) prevents programmed cell death in animal cells. We investigated the expression pattern of DAD‐1 in petals of iris ( Iris × hollandica cv. Blue Magic) and carnation ( Dianthus caryophyllus cv. Etarro). DAD‐1 expression in Iris petals was strongly reduced by the time of visible senescence, which occurs 4 days after flower opening. Microscopic analysis showed that most mesophyll cells had died prior to a clear decrease in DAD‐1 expression and that epidermis cells started to die by that time. In carnation petals DAD‐1 expression also decreased by the time of massive cell death. After ethylene treatment, DAD‐1 expression in carnation again decreased concomitant with the advance in massive cell death. In conclusion, DAD‐1 is not an early regulator of petal cell death. Its expression may be required for the programmed dismantling of cells, as it ceases only just prior to, or concomitant with, cell death.