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Disease stress‐inducible genes of tobacco: expression profile of elicitor‐responsive genes isolated by subtractive hybridization
Author(s) -
Takemoto Daigo,
Yoshioka Hirofumi,
Doke Noriyuki,
Kawakita Kazuhito
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2003.00145.x
Subject(s) - suppression subtractive hybridization , biology , methyl jasmonate , elicitor , nicotiana tabacum , pseudomonas syringae , gene , wrky protein domain , complementary dna , systemic acquired resistance , pathogenesis related protein , tobacco mosaic virus , gene expression , microbiology and biotechnology , cdna library , genetics , arabidopsis , transcriptome , virus , mutant
In order to investigate the change in mRNA profile during tobacco disease response, a subtractive hybridization procedure was used to generate a cDNA library for genes induced in tobacco ( Nicotiana tabacum cv. Samsun NN) treated with oomycete elicitor. Database searches with the randomly isolated genes revealed that this cDNA library was enriched for reported disease stress‐responsive genes such as pathogenesis‐related proteins and cell wall protein genes. The expressions of eight newly isolated genes were induced by inoculation with the non‐pathogenic bacteria, Pseudomonas syringae pv. glycinea . The NtEIG s ( N . t abacum e licitor‐ i nducible g enes) showed similarity to genes for stellacyanin‐like protein ( NtEIG‐A1 ), glutathione peroxidase ( NtEIG‐C08 ), extensin‐like protein ( NtEIG‐C29 ), WRKY transcription factor ( NtEIG‐D48 ), glycine rich protein ( NtEIG‐E17 ), β ‐1, 3‐glucanase‐like protein ( NtEIG‐E76 ), photoassimilate‐responsive protein‐1 ( NtEIG‐E80 ) and wound‐induced protein ( NtEIG‐D10 ). The expression patterns of NtEIG s in tobacco leaf in response to P. syringae pv. glycinea , salicylic acid (SA), methyl jasmonate (MeJA) and wound stress were analysed. The individual expression patterns of NtEIG s indicate that the transcriptional activation of NtEIG s is regulated by various signals and the products of NtEIG s are involved in different processes at different stages of the plant defence responses.