Premium
Adventitious shoot regeneration from Begonia × erythrophylla petiole sections is developmentally sensitive to light quality
Author(s) -
Burritt David J.,
Leung David W. M.
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2003.00083.x
Subject(s) - phytochrome , shoot , explant culture , petiole (insect anatomy) , primordium , botany , biology , far red , begonia , red light , blue light , organogenesis , basal shoot , horticulture , in vitro , biochemistry , optics , hymenoptera , physics , gene
The influence of light quality on organogenesis in vitro was investigated using Begonia × erythrophylla petiole explants. Pre‐treatment of in vitro donor plants by growth in the dark or under far‐red or blue light reduced their competence for shoot formation when compared with those grown under red or white light. Culture of competent petiole explants under far‐red, blue light or in the dark reduced the number of shoots produced per explant compared to those cultured under red or white light. Explants were found to be developmentally sensitive to both far‐red and blue light, because meristem, but not primordia development was inhibited. In addition, blue light inhibition of shoot formation is not mediated directly through phytochrome, as few shoots formed on explants cultured under a mixture of red and blue light which resulted in a high P fr / P tot (0.82) and would allow shoot formation in the absence of blue light. Unlike the inhibitory influence of far‐red light, which is reversible, exposure to blue light permanently reduces an explant's competence for shoot formation. Our results suggest that phytochrome and an independent blue light photoreceptor, possibly a cryptochrome, can regulate shoot production from B. erythrophylla petiole explants.