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Measurements of photosynthesis and respiration in plants
Author(s) -
Hunt Stephen
Publication year - 2003
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2003.00055.x
Subject(s) - photosynthesis , respiration , chlorophyll fluorescence , carbon fixation , biological system , gas phase , quantum yield , fluorescence , chemistry , environmental science , botany , biology , physics , biochemistry , optics
Methods for measuring the rates of photosynthesis and respiration in plants are reviewed. Closed systems that involve manometric techniques, 14 CO 2 fixation, O 2 electrodes and other methods for measuring dissolved and gas phase O 2 are described. These methods typically provide time‐integrated rate measurements, and limitations to their use are discussed. Open gas exchange systems that use infra‐red CO 2 gas analysers and differential O 2 analysers for measuring instantaneous rates of CO 2 and O 2 exchange are described. Important features of the analysers, design features of gas exchange systems, and sources of potential error are considered. The analysis of chlorophyll fluorescence parameters for estimating the quantum yield for O 2 evolution and CO 2 fixation is described in relation to new fluorescence imaging systems for large scale screening of photosynthetic phenotypes, and the microimaging of individual chloroplasts.