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Photoregulation of phenylalanine ammonia‐lyase (PAL) and chalcone synthase (CHS) in the accumulation of anthocyanin in alpine and prairie ecotypes of Stellaria longipes under varied R/FR
Author(s) -
Alokam Suneetha,
Li Yujing,
Li Wenze,
Chinnappa C. C.,
Reid David M.
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1160412.x
Subject(s) - ecotype , chalcone synthase , phenylalanine ammonia lyase , anthocyanin , phenylalanine , biology , botany , enzyme assay , enzyme , biochemistry , biosynthesis , amino acid
We studied mRNA accumulation and enzyme activity of phenylalanine ammonia‐lyase (PAL) and chalcone synthase (CHS) in alpine and prairie ecotypes of Stellaria longipes . The results show that light is required for the induction of PAL and CHS and accumulation of anthocyanins. Although both the ecotypes showed higher levels of anthocyanins under high R/FR (1.9) than under low R/FR (0.7), neither of the ecotypes showed any significant difference in the transcript abundance of PAL or CHS between low R/FR and high R/FR. However, both ecotypes showed significantly higher levels of CHS activity under high R/FR than under low R/FR corresponding to their anthocyanin accumulation. These results indicate that in S. longipes regulation of CHS activity is more specific to anthocyanin synthesis than PAL under varied R/FR, with a time lag between the enzyme activity and the accumulation of anthocyanins.