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Anthocyanins facilitate tungsten accumulation in Brassica
Author(s) -
Hale Kerry L.,
Tufan Hale A.,
Pickering Ingrid J.,
George Graham N.,
Terry Norman,
Pilon Marinus,
PilonSmits Elizabeth A. H.
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1160310.x
Subject(s) - anthocyanin , brassica rapa , brassica , tungstate , brassica oleracea , chemistry , botany , horticulture , food science , biology , organic chemistry
Accumulation of molybdenum in Brassica was recently found to be correlated with anthocyanin content, involving the formation of a blue complex. Here the role of anthocyanins in tungsten sequestration was investigated using three species of Brassica : B. rapa (cv. Fast plants), B. juncea (Indian mustard) and B. oleracea (red cabbage). Seedlings of B. rapa and B. juncea turned blue when supplied with colourless tungstate. The blue compound co‐localized with anthocyanins in the peripheral cell layers, and the degree of blueness was correlated with anthocyanin content. The direct involvement of anthocyanins in the blue coloration was evident when purified anthocyanins showed a colour change from pink to blue in vitro upon addition of tungstate, over a wide pH range. Anthocyanin production was upregulated 3‐fold by W in B. juncea , possibly reflecting a function for anthocyanins in W tolerance or sequestration. The presence of anthocyanins facilitated W accumulation in B. rapa : anthocyanin‐containing seedlings accumulated 3‐fold more W than an anthocyaninless mutant. There was no correlation between anthocyanin content and W tolerance under these conditions. The nature of the interaction between anthocyanins and tungstate was investigated. X‐ray absorption spectroscopy showed no change in the local chemical environment of W upon uptake of tungstate by the plant; HPLC analysis of purified anthocyanin with or without tungstate showed no peak shift after metal treatment.