Premium
Evidence for the direct 2 β ‐ and 3 β ‐hydroxylation of [ 2 H 2 ]GA 20 ‐13‐O‐[6′‐ 2 H 2 ]glucoside in seedlings of Phaseolus coccineus
Author(s) -
Schneider Gernot,
Fuchs Petra,
Schmidt Jürgen
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1160202.x
Subject(s) - glucoside , hydroxylation , gibberellin , chemistry , stereochemistry , enzyme , biochemistry , botany , biology , medicine , alternative medicine , pathology
[17‐ 2 H 2 ]GA 20 ‐13‐O‐[6′‐ 2 H 2 ]glucoside was synthesized and applied to seedlings of Phaseolus coccineus L. After incubation for 72 h the conjugate metabolites were purified and shown by LC‐ESI‐tandem‐MS and GC‐MS to be [17‐ 2 H 2 ]GA 1 ‐13‐O‐[6′‐ 2 H 2 ]glucoside and [17‐ 2 H 2 ]GA 29 ‐13‐O‐[6′‐ 2 H 2 ]glucoside. This is the first evidence for the conversion of intact GA‐O‐glucosides, and represents an additional metabolic pathway of the gibberellin metabolism in P. coccineus L. The results indicate that intact GA‐O‐glucosides are accepted by 2‐ and 3‐oxidases in the plant.