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Vernalization, photoperiod and GA 3 interact to affect flowering of Japanese radish ( Raphanus sativus Chinese Radish Jumbo Scarlet)
Author(s) -
Erwin John E.,
Warner Ryan M.,
Smith Alan G.
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1150217.x
Subject(s) - vernalization , raphanus , photoperiodism , biology , horticulture , bolting , botany , gibberellin , brassica
Raphanus sativus L. Chinese Radish Jumbo Scarlet has characteristics that make it an excellent plant model for vernalization studies. This study further characterizes flower induction of R. sativus Chinese Radish Jumbo Scarlet. Seed were imbibed in distilled water containing 0, 10 −5 M or 10 −3 M GA 3 for 24 h and were then exposed to 6 ± 0.5°C (vernalized) for 0, 2, 4, 6, or 8 days. Seedlings were then grown under a short‐ (8 h) or long‐day photoperiod (8 h with or without a 4‐h night interruption; 2200–0200 h). Of unvernalized plants grown under long‐ and short‐day conditions, 45 and 3% flowered, respectively. Saturation of the vernalization response occurred after a 4‐ or 8‐day vernalization treatment when plants were placed under long‐ or short‐days, respectively. Basal leaf number and days to anthesis decreased when seeds were cooled for 2 or 4 days and were imbibed with 10 −3 M GA 3 compared to distilled water only. These data indicate that R. sativus Chinese Jumbo Scarlet has principally an obligate vernalization requirement when grown under short‐days. GA 3 application only facilitated flowering when the length of the vernalization treatment was marginal. Taken together, these data support the use of this plant as a model plant for identifying vernalization responses under short‐day conditions.