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Cloning of nodule‐specific cDNAs of Galega orientalis
Author(s) -
Kaijalainen Seppo,
Schroda Michael,
Lindström Kristina
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1140412.x
Subject(s) - cloning (programming) , biology , botany , genetics , computer science , programming language
Differential display was applied in order to clone cDNAs expressed exclusively or predominantly in nodules, compared to uninoculated root tissue of Galega orientalis . Forty‐five fragments were unique for nodule RNA. These fragments were reamplified and cloned. Six of them produced a nodule‐specific signal on Northern hybridization. These six fragments were sequenced. Five of the sequenced fragments showed homology to nodulin‐gene sequences in databases, among them Vicia faba mRNA for protein showing partial homology with Medicago sativa nodulin‐25 (Nms25), Pisum sativum PsN466, V. faba CCP2 and CCP4, P. sativum ENOD3 , and Maackia amurensis ENOD2. The remaining sequence had no significant homology with sequences in the databanks. Full‐size cDNA for the homologue to V. faba mRNA for the protein showing partial homology with M. sativa nodulin‐25 (Nms25) and P. sativum PsN466 were cloned and sequenced.