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Retardation and inhibition of the cation‐induced superoxide generation in BY‐2 tobacco cell suspension culture by Zn 2+ and Mn 2+
Author(s) -
Kawano Tomonori,
Kawano Nakako,
Muto Shoshi,
Lapeyrie Frédéric
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1140309.x
Subject(s) - superoxide , chemistry , reactive oxygen species , nicotiana tabacum , nadph oxidase , extracellular , oxidative stress , respiratory burst , biochemistry , nuclear chemistry , enzyme , gene
Salts at high concentrations may cause oxidative damage to plant cells since many studies indicated the involvement of reactive oxygen species in salt‐stress response. Recently, we have demonstrated that treatment of tobacco ( Nicotiana tabacum ) cell suspension culture with various salts result in an immediate burst of superoxide production via activation of NADPH oxidase by ions of alkali metals (Li + , Na + , K + ), alkali earth metals (Mg 2+ , Ca 2+ ) or lanthanides (La 3+ , Gd 3+ ). In this study, we tested the effect of extracellular supplementation of Zn 2+ and Mn 2+ on the cation‐induced oxidative burst in tobacco cell suspension culture, measured with a superoxide‐specific Cypridina luciferin‐derived chemiluminescent reagent. Extracellular supplementation of Zn 2+ and Mn 2+ inhibited the generation of superoxide in response to addition of salts. Although both Zn 2+ and Mn 2+ inhibited the salt‐induced generation of superoxide, the modes of inhibition by those ions seemed to be different since Mn 2+ simply inhibited total production of superoxide while Zn 2+ inhibited the early phase of superoxide production and induced the slow release of superoxide. Roles of Mn 2+ and Zn 2+ in protection of plant cells from salt stress, as an effective superoxide scavenger and an effective inhibitor of plasma membrane‐bound NADPH oxidase, respectively, are discussed.