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Steady‐state chlorophyll fluorescence (Fs) measurements as a tool to follow variations of net CO 2 assimilation and stomatal conductance during water‐stress in C 3 plants
Author(s) -
Flexas Jaume,
Escalona José Mariano,
Evain Sebastian,
Gulías Javier,
Moya Ismaël,
Osmond Charles Barry,
Medrano Hipólito
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1140209.x
Subject(s) - stomatal conductance , chlorophyll fluorescence , photosynthesis , conductance , vitis vinifera , irradiance , fluorescence , chemistry , water stress , quenching (fluorescence) , chlorophyll , chlorophyll a , electron transport chain , botany , analytical chemistry (journal) , environmental science , environmental chemistry , biology , optics , physics , condensed matter physics
Water stress experiments were performed with grapevines ( Vitis vinifera L.) and other C 3 plants in the field, in potted plants in the laboratory, and with detached leaves. It was found that, in all cases, the ratio of steady state chlorophyll fluorescence (Fs) normalized to dark‐adapted intrinsic fluorescence (Fo) inversely correlated with non‐photochemical quenching (NPQ). Also, at high irradiance, the ratio Fs/Fo was positively correlated with CO 2 assimilation in air, with electron transport rate calculated from fluorescence, and with stomatal conductance, but no clear correlation was observed with qP. The significance of these relationships is discussed. The ratio Fs/Fo, measured with a portable instrument (PAM‐2000) or with a remote sensing FIPAM system, provides a good method for the early detection of water stress, and may become a useful guide to irrigation requirements.