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Accumulation of a 31‐kDa glycoprotein in association with the expression of embryogenic potential by spinach callus in culture
Author(s) -
Ishizaki Takuma,
Megumi Chiaki,
Komai Fuminori,
Masuda Kiyoshi,
Oosawa Katsuji
Publication year - 2002
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2002.1140115.x
Subject(s) - spinach , callus , gibberellic acid , spinacia , biology , isoelectric point , biochemistry , somatic embryogenesis , molecular mass , amino acid , polyacrylamide gel electrophoresis , chenopodiaceae , microbiology and biotechnology , botany , tissue culture , in vitro , germination , gene , chloroplast , enzyme
Calli grown from segments of spinach ( Spinacia oleracea L.) root in the presence of gibberellic acid (GA 3 ) plus auxin, differentiated to yield somatic embryos after transfer to a medium without growth regulators, while calli formed in the absence of GA 3 failed to generate any embryos. We extracted proteins from the two types of callus and analysed them by polyacrylamide gel electrophoresis. Compared with the proteins from calli formed on medium that contained only naphthaleneacetic acid (NAA) as a growth regulator, the proteins from calli grown in the presence of GA 3 included appreciably higher levels of a 31‐kDa basic protein (pI = 8.8). The protein resembled type I ribosome‐inactivating proteins (EC 3.2.2.22) in terms of molecular mass, isoelectric point, sequence of amino‐terminal amino acids and extent of glycosylation. The 31‐kDa protein was barely detectable in extracts of various tissues from seedlings. Thus, it is possible that an increase in the relative level of this protein might be associated with the expression of embryogenic potential expressed by spinach callus.