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Boron compartmentation in roots of sunflower plants of different boron status: A study using the stable isotopes 10 B and 11 B adopting two independent approaches
Author(s) -
Pfeffer Heidrun,
Dannel Frank,
Römheld Volker
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1130307.x
Subject(s) - vacuole , cytosol , intracellular , boron , cell wall , sunflower , isotopes of boron , apoplast , efflux , biology , biochemistry , chemistry , horticulture , enzyme , cytoplasm , organic chemistry
The intracellular compartmentation of boron (B) in roots of sunflower plants precultured with 100 μ M B (high B) or 1 μ M B (low B) was studied using two independent approaches. In the first approach, short‐term efflux studies using the stable isotopes 11 B and 10 B were carried out. In roots of high B plants, the calculated concentrations of B (nmol g FW −1 ) were 52.6 in the cell wall, 7.5 in the vacuole, 27.1 in the cytosol and 48.0 in the free space. In roots of low B plants, the concentrations of B (nmol g FW −1 ) were 43.4 in the cell wall, 2.8 in the vacuole, 17.9 in the cytosol and almost zero in the free space. Although the B supply differed by a factor 100, the B concentrations in the cytosol and the vacuole of low B plants were 66 and 37% of the respective concentrations in high B plants. This suggests an additional role for B in plant metabolism, besides its function in the cell wall. In the second approach, root B pools (cell sap and water‐insoluble residue) were determined for comparison, and found to be in good agreement with the results from the efflux study.