Premium
LeGRP1: A new member of glycine‐rich proteins from tomato ( Lycopersicon esculentum )
Author(s) -
Uthappa Mukatira,
Muchhal Umesh S.,
Baldwin James C.,
Raghothama K. G.
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1130112.x
Subject(s) - biology , glycine , open reading frame , lycopersicon , signal peptide , gene , complementary dna , peptide sequence , glycine cleavage system , biochemistry , amino acid , gene expression , microbiology and biotechnology , botany
Glycine‐rich proteins (GRPs) are members of a family of proteins with unique repeats of glycine‐containing motifs. This feature facilitates their association with plant structural components such as cell wall and vascular tissues. A transcript encoding a new member ( LeGRP1 ) of the GRP family was identified from roots of tomato by the method of differential display of mRNA species. The cDNA is 1.15 kb in size and has an open reading frame encoding a peptide of 284 amino acids. Glycine residues make up 55% of the deduced peptide and the sequence is characterized by repeating (Gly‐X) n domains. The presence of a hydrophobic N‐terminus with a putative signal peptide indicates a probable association of the protein with the cell wall. Southern analysis shows that several LeGRP1 homologues are present in the genome. The LeGRP1 transcript accumulates predominantly in the roots, stem and petioles of tomato. A relatively lower level of LeGRP1 message was observed in the leaf mid‐rib, while the message is undetectable in the leaf lamina. Abiotic stresses like cold, heat, salt, desiccation and phosphate starvation did not significantly affect the expression of LeGRP1 , whereas wounding resulted in a significant repression of the gene expression . Temporal regulation of LeGRP1 was observed during fruit development.