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Small complex‐type N‐linked glycans are attached to cell‐wall bound exo‐ β ‐glucanases of both mung bean and barley seedlings
Author(s) -
Kotake Toshihisa,
Tonari Akiko,
Ohta Masaya,
Matsuura Fumito,
Sakurai Naoki
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1120302.x
Subject(s) - cell wall , glycan , mung bean , chemistry , biochemistry , acetylation , glycoprotein , food science , gene
N‐linked glycans of wall‐bound exo‐ β ‐glucanases from mung bean and barley seedlings, namely Mung‐ExoI and Barley‐ExoII, were characterized. The N‐linked glycans of Mung‐ExoI and Barley‐ExoII were liberated by gas‐phase hydrazinolysis followed by re‐N‐acetylation. Their structures were determined by two‐dimensional sugar‐mapping analysis and MALDI‐TOF mass spectrometry. N‐glycans from both glucanases were of paucimannosidic‐type (small complex‐type) structures, Man α 1‐6(±Man α 1‐3)(Xyl β 1‐2)Man β 1‐4GlcNAc β 1‐4(±Fuc α 1‐3) GlcNAc, which are known as typical vacuole‐type N‐glycans. The results suggest that N‐glycans of cell‐wall glucanase were produced by partial trimming of complex‐type N‐glycans by exoglycosidases during its transport from Golgi apparatus to cell walls or in the cell walls.