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Temporal and tissue localization of a cowpea ( Vigna unguiculata ) cystatin
Author(s) -
Flores Victor M. Q.,
Louro Ricardo P.,
XavierFilho José,
Barratt Daniel H. P.,
Shewry Peter R.,
Fernandes Kátia V. S.
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1120207.x
Subject(s) - vigna , imbibition , cystatin , germination , antiserum , biology , blot , cotyledon , botany , cystatin c , biochemistry , antibody , immunology , renal function , gene
A cowpea ( Vigna unguiculata L. Walpers cv. Pitiúba) cystatin was analysed to determine its localization during development and germination of cowpea seeds, using western blotting with a specific antiserum. The pattern of immunoreactive proteins changed during development, with the major reactive bands present in dried seeds being mobilized after a 62‐h period of imbibition. Immunohistochemical analysis revealed that cowpea cystatin is distributed in both embryonic axes and cotyledons with the highest level being present in the outer cell walls of the adaxial surface of the cotyledons.