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Correlation of resistance and H 2 O 2 production in Ulmus pumila and Ulmus campestris cell suspension cultures inoculated with Ophiostoma novo‐ulmi
Author(s) -
De Rafael M. A.,
Valle T.,
Babiano M. J.,
Corchete P.
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1110411.x
Subject(s) - scopoletin , dutch elm disease , biology , phytoalexin , pathogen , inoculation , botany , spore , ulmaceae , ulmus pumila , plant disease resistance , microbiology and biotechnology , horticulture , biochemistry , gene , medicine , alternative medicine , pathology , resveratrol
The Dutch elm disease (DED) pathogen Ophiostoma novo‐ulmi Buissm. elicited the production of H 2 O 2 in cell suspension cultures of the resistant species Ulmus pumila L. This response was not observed in suspensions of the susceptible elm U. campestris Mill. H 2 O 2 production started after a lag time of 30–40 min following inoculation, peaked between 4 and 6 h and lasted up to 24 h. Treatment of the suspensions with exogenously added H 2 O 2 did not cause accumulation of the sesquiterpene phytoalexins mansonones nor of the coumarin scopoletin. Spore germination and growth of O. novo‐ulmi were significantly delayed with different amounts of H 2 O 2 (0.1–1 m M ). These results suggest that H 2 O 2 production is an inducible defence response which may contribute to DED resistance by delaying the growth of the pathogen at the earliest stages of infection. Whether H 2 O 2 is involved in other elm defence responses to the pathogen is presently unknown, but its production seems to be an independent event from phytoalexin formation.