Premium
Subcellular localization of β‐glucosidase in rye, maize and wheat seedlings
Author(s) -
Nikus Jeanette,
Daniel Geoffrey,
Jonsson Lisbeth M. V.
Publication year - 2001
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1034/j.1399-3054.2001.1110406.x
Subject(s) - coleoptile , vascular bundle , cytoplasm , biology , cell wall , shoot , plastid , guard cell , xylem , botany , plant cell , microbiology and biotechnology , biophysics , chloroplast , biochemistry , gene
The subcellular compartmentation of β ‐glucosidase was studied in rye, maize and wheat seedlings by immunocytochemical methods. For detection, we used a 10 nm gold‐labeled secondary antibody, and results were observed using transmission electron microscopy. In all three species, β ‐glucosidase was found in plastids, cytoplasm and cell walls. In rye, gold particles were seen on cell walls and cytoplasm in epidermal cells of the root tip and shoot, in bundle sheath cells of the shoot and in all cells, except the vascular bundle cells of the coleoptile. Gold labeling was also observed in plastids of the bundle sheath cells of rye shoot tips and in cortical cells of root tips. In wheat, gold labeling was observed on cell walls and cytoplasm of epidermal cells in the shoot base and coleoptile, and on cell walls and plastids in epidermal cells of the root tip. In maize, gold labeling was mainly found in plastids or proplastids in vascular bundle cells and bundle sheath cells of the shoot, in bundle sheath cells of the coleoptile and in epidermal cells of the root. Some gold particles were also found in cell walls and cytoplasm of stomatal guard cells of the shoot base and vascular bundle cells of the shoot tip and in the cell walls of bundle sheath cells of the shoot tip and root tip epidermal cells. Results are discussed in relation to the role of β ‐glucosidase in hydroxamic acid release and overall defense mechanism of monocotyledons.